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Lysis buffer. A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells (e.g. western blot for protein, or for DNA extraction ).
SDS-PAGE is an electrophoresis method that allows protein separation by mass. The medium (also referred to as ′matrix′) is a polyacrylamide-based discontinuous gel. The polyacrylamide-gel is typically sandwiched between two glass plates in a slab gel. Although tube gels (in glass cylinders) were used historically, they were rapidly made ...
Sodium dodecyl sulfate ( SDS) or sodium lauryl sulfate ( SLS ), sometimes written sodium laurilsulfate, is an organic compound with the formula CH3(CH2)11OSO3Na and structure H 3 C − (CH 2) 11−O− S (=O) 2−O−Na+. It is an anionic surfactant used in many cleaning and hygiene products. This compound is the sodium salt of the 12-carbon ...
Safety data sheet. A safety data sheet ( SDS ), [1] material safety data sheet ( MSDS ), or product safety data sheet ( PSDS) is a document that lists information relating to occupational safety and health for the use of various substances and products. SDSs are a widely used type of fact sheet used to catalogue information on chemical species ...
The stock solution for Karnovsky fixative is as follows: [1] Mix the paraformaldehyde with 25 ml of distilled water in a 125 ml Erlenmeyer flask. Heat to 60 °C on a stir plate. When moisture forms on the sides of flask, add sodium hydroxide and stir until the solution clears. Cool solution under the faucet.
Dimethyl sulfoxide ( DMSO) is an organosulfur compound with the formula ( CH 3) 2 S O. This colorless liquid is the sulfoxide most widely used commercially. It is an important polar aprotic solvent that dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water.
Hoagland solution. The Hoagland solution is a hydroponic nutrient solution that was newly developed by Hoagland and Snyder in 1933, [1] modified by Hoagland and Arnon in 1938, [2] and revised by Arnon in 1950. [3] It is one of the most popular standard artificial solution compositions for growing plants, in the scientific world at least, with ...
In biochemistry and molecular biology, saline-sodium citrate (SSC) buffer is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray or Northern blotting. 20X SSC may be used to prevent drying of agarose gels during a vacuum transfer. A 20X stock solution ...