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The MycoAlertTM mycoplasma detection kit is a generic biochemical test for mycoplasma and other mollicutes (e.g. acholeplasmas, mesoplasmas, spiroplasmas) and will detect mollicutes of mammalian, avian, insect and plant origin. The following 44 mollicute species were tested using the MycoAlertTM assay.
Introduction. Bone marrow contains a population of rare progenitor cells known as mesenchymal stem cells (MSC) capable of replication as undifferentiated cells or differentiating into bone, cartilage, fat, muscle, tendon and marrow stroma. PoieticsTM human mesenchymal stem cell (hMSC) system contains normal human mesenchymal stem cells and ...
Thawing of cells / initiation of culture process. The recommended seeding density for adipose derived stem cells is 5,000 cells per cm2. To set up cultures, calculate the number of vessels needed based on the recommended seeding density and the surface area of the vessels being used.
All trademarks herein are marks of Lonza Group or its subsidiaries. Page 2 of 6 5. Remove the cryovial immediately, wipe it dry, and transfer to a sterile field where the equilibrated flasks should be waiting, ready to seed. Rinse the cryovial with 70% alcohol, and then wipe to remove excess. 6. Using a micropipette, gently add the thawed cell
PoieticsTM human ADSCs, available from normal or diabetic donors, are isolated from human lipoaspirate and cryopreserved from primary cultures. PoieticsTM adipose-derived stem cells and medium are quality tested together and guaranteed to give optimum performance as a complete cell system.
All trademarks herein are marks of Lonza Group or its subsidiaries. Page 2 of 4 3. Rinse each cryovial with the medium. It may not be possible to recover the entire volume listed for each cryovial. Small losses, even up to 10%, should not affect the cell growth characteristics of the supplemented medium. 4.
The recommended subculturing reagents for these cells are trypsin/EDTA (CC-5012), trypsin neutralizing solution (CC-5002), and HEPES buffered saline solution (CC-5022). These reagents can be purchased individually or together as part of the ReagentPackTM subculture reagents (CC-5034).
X-VIVOTM 20 Medium was developed and optimized to support the generation of LAK cells from monocyte-depleted PBL at high density. Initial cell densities between 2.0- 3.0 x 107 cells/ml were successfully used to generate LAK cells. X-VIVOTM Medium 20 may also be used as a growth medium for PBL and TIL.
Unpacking and storage instructions. Check all containers for leakage or breakage. For cryopreserved cells – remove cryovials from the dry ice packaging and immediately place into liquid nitrogen storage. Alternatively, thaw and use the cells immediately. If no dry ice remains, please contact customer service.
Luminometer Quick Start Guide. Supporting operating instructions for the LucettaTM Luminometer are available on the accompanying CD or at www.lonza.com/lucetta. Full kit instructions can be downloaded from www.lonza.com/toxilight.